PKRRATING SECRETS

3B). R526 from your loop concerning αJ and αI anchors the C-terminal percentage of the activation loop by forming a salt bridge with E458 at The bottom of αEF. Q459 stabilizes the HRD motif by a hydrogen bond to the principle chain carbonyl of R413. The tip in the activation segment is stabilized by a hydrogen bond amongst Y454 and E480 from αF